After screening out the compounds showing potential activity against the target through the hit identification stage, it is necessary to carry out further characterization and optimization of these hit compounds. And this stage is termed as hit to lead. The goal of this stage is to identify the most promising hit series through limited structure-activity relationship (SAR) studies to enter the lead optimization and preclinical development stage. With a dedicated team of skilled scientists and technicians, Creative Biostructure provides MagHelix™ hit evaluation and lead identification (hit to lead) solutions to meet our clients’ needs and goals. hit to lead in drug discovery https://drug-discovery.creative-biostructure.com/hit-to-lead-p44

Lead discovery and optimization play a crucial role to determine the quality of clinical candidates. After this process, a set of compounds can be screened that interact with the target and the confirmed hits have specific activity against the target. Creative Biostructure performs comprehensive lead discovery and optimization solutions including library construction, library screening (in silico, in vitro and in vivo), hit-to-lead optimization and preclinical evaluation, etc. Lead discovery services https://drug-discovery.creative-biostructure.com/lead-discovery-and-optimization-p19

Computer-aided drug design (CADD) has penetrated into all aspects of drug discovery, and with the in-depth application of artificial intelligence and cloud computing, it is foreseeable that CADD will play a greater role in drug discovery projects in the future, improving efficiency and further reducing high costs of drug development. Creative Biostructure's CADD and virtual screening platform is a comprehensive platform for new drug discovery that integrates a variety of computer applications. It can be utilized for early preclinical research of small molecule drugs, peptides, antibodies, and therapeutic proteins. In Silico Drug Discovery Service https://drug-discovery.creative-biostructure.com/maghelix-cadd-platform-p59

After screening out the compounds showing potential activity against the target through the hit identification stage, it is necessary to carry out further characterization and optimization of these hit compounds. And this stage is termed as hit to lead. The goal of this stage is to identify the most promising hit series through limited structure-activity relationship (SAR) studies to enter the lead optimization and preclinical development stage. With a dedicated team of skilled scientists and technicians, Creative Biostructure provides MagHelix™ hit evaluation and lead identification (hit to lead) solutions to meet our clients’ needs and goals. Hit Confirmation https://drug-discovery.creative-biostructure.com/hit-to-lead-p44

The analysis of three-dimensional (3D) structure is of great importance for the mechanism of virus infection and the design of antiviral drugs. Although many breakthroughs have been made in structural biology techniques and structure determination methods such as X-ray crystallography, NMR spectroscopy and cryo-electron microscopy (cryo-EM) have been utilized to determine the structure of many biological macromolecules, the process of obtaining the 3D structure of the target protein is still difficult and time-consuming. Facing the continuous spread of the epidemic, we need to accelerate the understanding of the pathogenic mechanism of the virus. Creative Biostructure can generate structural models of coronavirus/host proteins through calculation methods, which will be a powerful alternative and supplement to the experimental method. Coronavirus Homology Modeling https://www.creative-biostructure.com/coronavirus/protein-homology-modeling-for-coronavirus-research-p18.htm

The necessary condition for successful X-ray crystallographic analysis is to obtain a single crystal of the target protein that meets the X-ray diffraction standard. The data are then collected by X-ray diffraction from a single crystal with an ordered pattern of atomic orientation. The assembly of atoms and molecules in the crystal can be deduced from the measurement of X-ray scattering. As a senior supplier in the field of structural biology contract research, Creative Biostructure provides protein crystallization and optimization screening services to assist in the research of coronavirus infection. SARS-CoV-2 Crystal optimization https://www.creative-biostructure.com/coronavirus/protein-crystallization-and-optimization-screening-p25.htm

High-resolution microscopy is essential for visualizing viral particles, and the acquisition of morphological characteristics of viral particles depends on the resolution. Transmission electron microscopy (TEM) is the starting point for obtaining the optimal image resolution. Negative staining electron microscopy (EM) of virus suspensions can provide detailed information on the architecture of viral particles. This is a technique that can be performed rapidly and can adapt to the highest magnification of viral particles. It also provides an initial model for structural analysis of cryo-electron microscopy (cryo-EM). Creative Biostructure is a leading supplier in the field of structural biology and offers you negative staining EM services for the visualization of coronavirus-like particles. Coronavirus-like Particle Negative Staining EM https://www.creative-biostructure.com/coronavirus/negative-staining-electron-microscopy-for-coronavirus-like-particle-p51.htm

Obtaining the structural information of the complex of an individual protein with other molecules (such as proteins, nucleic acids, and small molecules) can help understand the mechanism underlying such interaction, which is significant for drug discovery and development. However, it is relatively difficult and costly to obtain three-dimensional (3D) complex structures through experimental methods such as X-ray crystallography or NMR spectroscopy. Nowadays, Creative Biostructure not only provides experimental techniques for structure determination, but also exploit computational docking methods to predict the 3D structures of these interacting partners to support the development of antiviral drugs against coronavirus infection. SARS-CoV-2 protein-protein docking https://www.creative-biostructure.com/coronavirus/molecular-docking-p30.htm

Creative Biostructure is specialized in providing cost-effective contract services to both academia and biotech/pharmaceutical industries in the field of structural biology and membrane protein technologies. We have developed all-in-one, gene-to-structure pipelines for the structure determination of macromolecules of your interest. With a team of experienced professionals, Creative Biostructure is able to solve the structure of many challenging proteins including GPCRs, ion channels, transporters, enzymes and viral targets. We also provide a comprehensive list of products and other related services to facilitate your research in structural biology. Exosome In vivo Imaging https://www.creative-biostructure.com/exosome-in-vivo-functional-assay.htm

Creative Biostructure is specialized in providing cost-effective contract services to both academia and biotech/pharmaceutical industries in the field of structural biology and membrane protein technologies. We have developed all-in-one, gene-to-structure pipelines for the structure determination of macromolecules of your interest. With a team of experienced professionals, Creative Biostructure is able to solve the structure of many challenging proteins including GPCRs, ion channels, transporters, enzymes and viral targets. We also provide a comprehensive list of products and other related services to facilitate your research in structural biology. Electron Microscopy data analysis https://www.creative-biostructure.com/em-data-processing-and-interpretation-585.htm

Electron Microscopy (EM) has become an extremely popular method for the ultrastructural study of macromolecules, cells, and tissues. An aqueous biological sample is frozen rapidly and irradiated with a beam of electrons. A detector senses how the electrons are scattered, and a computer reconstructs the 3D-shape of the molecule. Single-Particle Reconstruction https://www.creative-biostructure.com/cryo-em-services_4.htm

Cryo-electron microscopy (cryo-EM) is a form of transmission electron microscopy (TEM) where biological samples are studied at cryogenic temperatures. It is becoming a more and more important technique in the field of structural biology, as has been manifested by many once hard-to-determine macromolecular structures. With the rapid advancement of both hardware and software, the resolution of cryo-EM maps is improving steadily. In some cases, near-atomic resolution had been obtained, including those of viruses, ribosomes, mitochondria, ion channels, and enzyme complexes as small as 150 kD. Cryo-EM Small Protein https://www.creative-biostructure.com/cryo-em-for-small-proteins-561.htm