The product is produced by recombinant DNA technology using a non-pathogenic laboratory strain of Escherichia coli (K12) as the production organism. It is an analogue of human insulin made by replacing the asparagine residue at position A21 of the A-chain with glycine and adding two arginines to the C-terminus (positions B31 and 32) of the B-chain. The resulting protein is soluble at pH 4 and forms microprecipitates at physiological pH 7.4. C267H404N72O78S6 https://www.creativebiomart.net/therapeutic-proteins/p/127/human-insulin-analogue-replacing-the-asparagine-residue-at-position-a21-of-the-a-chain-with-glycine-and-adding-two-arginines-to-the-c-terminus-positions-b31-and-32-of-the-b-chain/