Creative Biostructure, a biotechnology company specialized in structural biology, now established a negative staining EM platform that can be used in biology, medicine, and drug discovery and development.

Negative staining and Native staining EM

Negative staining, also known as negative contrast staining, is a staining technique used for microscopic examination of opaque liquid specimens. In contrast to traditional dyeing, there is no reaction between the sample and the dye solution, so the sample is not colored. A white sample with a low electron density can be contrasted with a black background, forming an obvious negative contrast in contrast to the density difference between the sample and the dye. Negative staining electron microscopy (Negative staining EM) is widely used in sample analysis because it eliminates the need for complex procedures such as fixation, dehydration, embedding, and ultra-thin slicing.

With extensive expertise and experience in negative stain EM technology, Creative Biostructure now provides services that cover the entire technical process, including sample preparation, EM data acquisition and analysis.

Sample preparation service

Because there are numerous factors that influence the negative dyeing of samples, such as sample concentration, dye solution concentration and PH value, dyeing time and temperature, and so on, the scientific team at Creative Biostructure will assist customers in selecting the best conditions to prepare samples.

Data acquisition and analysis service

To inspect the quality of prepared samples, a transmission electron microscope with a low voltage (120 kV or 100 kV) is used. After good samples have been obtained, sample images will be collected. Using specialized software such as RELION, the particles were pretreated and selected, and the conformation of the target complex with low resolution was obtained through rounds of 2D average classification and optimization.

Creative Biostructure also offers cryo-EM-related research technologies such as single particle analysis, electron crystallography, microcrystal electron diffraction, and cryo-electron tomography.

Visit https://www.creative-biostructure.com/negative-staining-transmission-electron-microscopy-service.htm to know more about the negative staining EM platform provided by Creative Biostructure.