Creative BioMart, a biotechnology company that supplies reagents for the science community, recently enhanced its bacterial expression services, a proof of its goal to provide advanced tools for protein expression and purification research.


Escherichia coli (E. coli) was discovered by Escherich in 1885. Escherichia coli is an opportunistic pathogenic bacterium that can cause infections in various tissues and organs such as the gastrointestinal tract/urethra of humans and various animals under certain conditions. Escherichia coli belongs to the coliform-type anaerobic Gram-negative bacteria that usually exist in the intestinal tract of organisms. Most E. coli strains are harmless, but certain serotypes can cause severe food poisoning in the host.


The harmless E. coli is part of the normal gut flora and lives in symbiosis with this organism, which can benefit the body by producing vitamin K2 and prevent the spread of pathogens in the gut. Escherichia coli is a useful host strain for recombinant protein production. It has the advantages of clear genetic background, simple culture operation, high transformation and transduction efficiency, fast growth and reproduction, low cost, and rapid mass production of target proteins. Moreover, the expression level of exogenous gene products is much higher than other gene expression systems, and the amount of expressed protein can exceed 30% of the total bacterial protein. Therefore, the E. coli expression system is a widely used protein expression system, and about 30% of commercially available recombinant protein products are produced by the E. coli system.


The E. coli expression platform from Creative BioMart gathers a variety of bacterial expression vectors with various N- and C-terminal tags and host strains to guarantee the selection of the most effective system. Regarding insoluble proteins, skilled PhD-level scientists at Creative BioMart have created a successful protein refolding system that consists of an improved refolding buffer and procedure. Regarding fermentation scale, the team has bioreactors that range in size from 5 to 20,000 liters to meet diversified needs.


The Bacillus subtilis system from Creative BioMart is a great choice for the high-level expression of monomeric protein products. When combined with its secret fermentation protocols, this system enables rapid process development and scale-up to support early clinical testing.


Other protein expression services are also available at Creative BioMart, covering: Baculovirus-Insect Cell expression Systems (SF9, SF21, S2, / HI-5), Cell-Free in Vitro Protein expression, Mammalian expression Systems (CHO / HEK293), Yeast expression Systems (P pastoris / S cerevisiae), High-throughput Protein Production, etc.


To know more detailed information about the bacterial protein expression service provided by Creative BioMart, please visit