N-terminal sequencing by Edman degradation, is a traditional method for sequencing protein, and still has advantages for protein analysis that cannot readily be obtained by other analysis methods. Edman degradation is a cyclic procedure: N-terminal amino acid residues is labeled and cleaved off from the peptides or proteins at a time, and is identified by chromatography. Generally, the N-terminal amino group of the protein is reacted with phenyl isothiocyanate to form a phenylthiocarbamoyl derivative. Under mildly acidic conditions, the derivative is released from the rest of the protein as a cyclic phenylthiohydantoin (PTH) amino acid. The released PTH amino acid is identified by high performance liquid chromatography (HPLC) or HPLC-MS. The remainder of the peptide is intact and available for another round of labeling and release. edman degradation protein https://www.creative-proteomics.com/pronalyse/n-terminal-sequencing-by-edman-degradation-service.html

Therapeutic proteins products have been proven effective against various diseases. Complex post-translational modifications (PTMs) are required by most therapeutic proteins. Common PTMs include glycosylation, oxidation, deamidation, proteolysis, and so forth. These PTMS not only affect efficient secretion, drug efficacy and stability of therapeutic proteins, but also may influence safety, immunogenicity, serum clearance, pharmacokinetics, etc. deamidation analysis https://www.creative-proteomics.com/pronalyse/deamidation-and-oxidation-analysis-service.html

Glycosylation, the process of attaching glycans to proteins or other organic molecules, is one of the most common post-translational modifications (PTMs). Glycans are highly branched carbohydrate structures, consisting of monosaccharide sugars, such as fucose, galactose, manose, sialic acid, and N-acetylglucosamine. Due to the complexity and diversity of composition and structure of glycans, and the site of glycan attachment, glycosylation may affect product stability, immunogenicity, serum clearance, pharmacokinetics, and anti-inflammatory activity. Therefore, glycosylation is a Critical Quality Attribute (CQA) that must be presented. Glycosylation analysis is critical step to ensure safety and potency of biopharmaceutical products during the process of drug discovery. glycosylation analysis https://www.creative-proteomics.com/pronalyse/glycosylation-sites-and-oligosaccharides-analysis-service.html

2D-Western Blot assay is a fast, simple and accurate biochemical technique for the analysis of protein drugs or peptides. The combination of 2D-WB and mass spectrometry can effectively analyze the characteristics of proteins and antigens in specific antibodies. western blot analysis https://www.creative-proteomics.com/pronalyse/2d-western-blot-assay-service.html

Protein glycosylation is a common post-translational modification of proteins, which transports glycans to proteins and specific amino acid residues to form glycosidic bonds under the action of glycosyltransferase. glycan analysis https://metabolomics.creative-proteomics.com/glycan-biosynthesis-and-metabolism-analysis.htm

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Phosphatidylethanolamine (PE) is the second most abundant phospholipid in mammalian cells. PE comprises about 15–25% of the total lipid in mammalian cells. It is enriched in the inner leaflet of membranes, and it is especially abundant in the inner mitochondrial membrane. phosphatidylethanolamine assay https://lipidomics.creative-proteomics.com/phosphatidylethanolamine.htm

Cellular membranes contains a distinct composition of various glycerophospholipids such as phosphatidic acid (PA), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylglycerol (PG), phosphatidylinositol (PI), cardiolipin (CL), lysophosphatidic acid (LPA) and lysobisphosphatidic acid (also known as bis(monoacylglycerol) hydrogen phosphate - BMP). glycerophospholipid https://lipidomics.creative-proteomics.com/glycerophospholipids-targeted-lipidomics.htm

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Leukotrienes (LTs), which are derived from arachidonic acid through two steps catalyzed by 5-lipoxygenase (5-LO), are inflammatory mediators that function in normal host defense and play roles in inflammatory diseases. The LTs are composed of cysteinylleukotrienes (CysLTs) and leukotriene B4 (LTB4); leukotriene https://lipidomics.creative-proteomics.com/leukotrienes-targeted-lipidomics.htm

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